A shell-less chick embryo culturing technique, reproduced successfully under local circumstances.
نویسندگان
چکیده
منابع مشابه
Optimized ex-ovo culturing of chick embryos to advanced stages of development.
Research in anatomy, embryology, and developmental biology has largely relied on the use of model organisms. In order to study development in live embryos model organisms, such as the chicken, are often used. The chicken is an excellent model organism due to its low cost and minimal maintenance, however they present observational challenges because they are enclosed in an opaque eggshell. In or...
متن کامل"Experiments on the Development of Chick and Duck Embryos, Cultivated in vitro" (1932), by Conrad Hal Waddington
Conrad Hal Waddington?s ?Experiments on the Development of Chick and Duck Embryos, Cultivated in vitro [5],? published in 1932 in Philosophical Transactions of the Royal Society of London, Series B, compares the differences in the development of birds [6] and amphibians [7]. Previous experiments focused on the self differentiation [8] of individual tissues in birds [6], but Waddington wanted to...
متن کاملA Chick Embryo in-Vitro Model of Knee Morphogenesis
Background: In this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. Methods: The developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of ...
متن کاملSupplemented eggshell restores calcium transport in chorioallantoic membrane of cultured shell-less chick embryos.
It was previously reported (Tuan, 1980a) that the development-specific expression of calcium transport and related functions in the chick embryonic chorioallantoic membrane (CAM) requires the continuous presence of the eggshell, the calcium source of the embryo. To further understand the mechanism of action of the eggshell on the CAM functions, this study reports the effects of eggshell supplem...
متن کاملEffect of experimentally induced calcium deficiency on development, metabolism and liver morphogenesis of the chick embryo.
To study the effects of systemic calcium deficiency on embryonic development, chick embryos maintained in long-term shell-less cultures were compared to control embryos incubated in ovo with respect to various parameters of metabolism and growth. After incubation day 14, retarded growth and development were apparent in shell-less embryos which exhibited severe hypocalcaemia and hyperphosphataem...
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ورودعنوان ژورنال:
- Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
دوره 18 9 شماره
صفحات -
تاریخ انتشار 2008